>>Extraction and quantification of metal-containing nanoparticles in marine shellfish based on single particle inductively coupled plasma-mass spectrometry technique
题名：Extraction and quantification of metal-containing nanoparticles in marine shellfish based on single particle inductively coupled plasma-mass spectrometry technique
来源：Journal of Hazardous Materials
发表年代：2022
作者：Yuan Sun, Yi Yang, Fei-Yun Tou, Zuo-Shun Niu, Xing-Pan Guo, Chang Liu, Jia Yan, Jia-Yuan Wu, Miao Xu, Li-Jun Hou, Min Liu
Abstract：Quantitative characterization of nanoparticles(NPs) in marine shellfish is critical to understanding the risks of bio-accumulation. Based on single particle(sp)ICP-MS and electron microscopy, a standardized protocol was developed to extract Ag, Au, and indigenous Ti-containing NPs from mussels. The optimal parameters are: dry sample extraction with tetramethylammonium hydroxide(TMAH), 5%(v/v) final concentration of TMAH,extraction at 25℃ for 12 h, and separation by centrifugation(3000 rpm for 5 min). The particle number recoveries of spiked Ag and Au NPs were 88±0.9% and 95±1.1%, respectively, while Ti-containing NPs had a particle number concentration of 8.2 x 106 particles/mg and an average size of 70 nm in tested mussels.Furthermore, titanium oxide NPs, including rutile, anatase, and Magnéli phases(TixO2x-1) were found ubiquitously in 10 shellfish based on the optimal method. The particle number concentrations and average sizes of the Ti-containing NPs were 2.1 x106-8.4 x106 particles/mg and 70-80 nm,respectively. These Ti-containing NPs,such as TiO2, accounted for about half of the Ti mass in shellfish, indicating that marine shellfish may be a significant sink for Ti-containing NPs.
